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Fig. 1 Immobilized Human Claudin 18.2 VLP at 5 μg/ml (100 μl/Well). Dose response curve for Anti-Claudin 18.2 Antibody, hFc Tag with the EC₅₀ of 9.8 ng/ml determined by ELISA.

Fig. 3 Immobilized Human Claudin 6 VLP at 5 μg/ml (100 μl/Well). Dose response curve for Anti-Claudin 6 Antibody, mFc Tag with the EC₅₀ of 2.5 ng/ml determined by ELISA.

Fig. 5 Immobilized Human GPRC5D Nanodisc, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC₅₀ of 4.9 ng/ml determined by

Fig. 7 Immobilized Human CD3E&CD3D, His Tag at 2 μg/ml (100 μl/well) on the plate, add serial dilutions of Anti-Human CD3×GPRC5D Bispecific Antibody, hFc Tag and then add Biotinylated Human GPRC5D Nanodisc, His Tag at 5 μg/ml. Detection was performed using HRP-conjugated streptavidin with the EC₅₀ of 0.28 μg/ml determined by ELISA.

Fig. 9 Immobilized Human CD3E&CD3D at 5 μg/ml (100 μl/well) on the plate, add serial dilutions of Anti-Human CD3XGPRC5D bispecific antibody in 60% human serum and then add various dilution ratio of biotinylated human GPRC5D VLP. Detection was performed using HRP-conjugated streptavidin with the EC₅₀ of 0.28 μg/ml determined by ELISA. The backcalculation bias is less than 20%, and the sensitivity of the method can reach 6.6 ng/ml.